DNA restriction and modification systems in Neisseria gonorrhoeae
نویسندگان
چکیده
منابع مشابه
The Neisseria gonorrhoeae S.NgoVIII restriction/modification system: a type IIs system homologous to the Haemophilus parahaemolyticus HphI restriction/modification system.
Strains of Neisseria gonorrhoeae possess numerous restriction-modification (R-M) systems. One of these systems, which has been found in all strains tested, encodes the S. NgoVIII specificity (5'TCACC 3') R-M system. We cloned two adjacent methyltransferase genes (dcmH and damH), each encoding proteins whose actions protect DNA from digestion by R.HphI or R.Ngo BI (5'TCACC 3'). The damH gene pro...
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Objective-To characterise Neisseria gonorrhoeae isolates by restriction fragment length polymorphisms (RFLPs) in ribosomal RNA genes. Design-Generation of RFLP patterns by HincII restriction of rRNA genes followed by hybridisation with a nonradioactive labelled broad spectrum 16 + 23S rRNA gene probe. This typing method was developed and compared with MAb based serotyping. Specimens-Forty three...
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Two plasmid-carried restriction-modification (R-M) systems, EcoRI (from pMB1 of Escherichia coli) and Bsp6I (from pXH13 of Bacillus sp. strain RFL6), enhance plasmid segregational stability in E. coli and Bacillus subtilis, respectively. Inactivation of the endonuclease or the presence of the methylase in trans abolish the stabilizing activity of the R-M systems. We propose that R-M systems med...
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A modification of the rapid sugar fermentation procedure has been developed because of difficulties encountered in reproducing the reported results. Changes in the method have made the test more sensitive and reproducible.
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Since the role of DNA restriction-modification (R-M) systems of hsd genes has been elucidated, recipient cells for unmodified or foreign DNA are usually restrictionless mutants. Such mutants may either modify and have the phenotype rm+ or fail to modify and have the phenotype rm-. Each R-M system consists of three genes: hsdS for specificity, hsdR for restriction, and hsdM for modification (1)....
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ژورنال
عنوان ژورنال: Clinical Microbiology Reviews
سال: 1989
ISSN: 0893-8512,1098-6618
DOI: 10.1128/cmr.2.suppl.s78